Review of Related Literature - 1454 Words

REVIEW OF RELATED LITERATURE This investigatory project aims to make use of rambutan peeling extracts that is high in phenolic compounds such as tannin and saponin that exhibits antioxidant activity and antibacterial activity against pathogenic bacteria. Although the seeds contain such compounds the peeling exhibit greater amount of the said compounds. Rambutan is a very popular fruit cultivated in the Philippines and it is eaten in large amounts. The rambutans rind is quickly discarded and thrown out after it is cut open and peeled off. The researchers will be maximizing the use of rambutan peelings through this project before they are disposed of. Not only will the researchers maximize the use of the peelings, they would be finding†¦show more content†¦It is the most common bacteria that causes staph infections such as pimples, boils, impetigo, cellulitis folliculitis, carbuncles, scalded skin syndrome and abscesses. Examples of the more serious sicknesses are pneumonia,me ningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS), bacteremia and septicemia. Like the other listed pathogenic bacteria, Staphylococcus epidermidis is also nosocomial and facultatively anaerobic. It affects the skin and is non hemolytic, hemolytic meaning relating to the rupture or destruction of red blood cells. It infects on people with weak immune systems. The infections often start out as skin wounds caused by flexible tubes called catheters. According to Bukhari, â€Å"Catheter infections along with catheter-induced UTIs lead to serious inflammation and pus secretion. In these instances, urination is extremely painful.† Other diseases of Staphylococcus epidermidis are septicaemia and endocarditis. The study of Qarah, Cunha and Dua in 2009 stated that pseudomonal pathogens evidently inhabit nature like soil, water and surfaces of plants and animals, including us humans. Pseudomonas aeruginosa is widely infectious especially to those with problems involving their host defense mechanisms. It frequently causes nosocomial infections such as pneumonia, urinary tract infections (UTIs), and bacteremia. This pathogenic bacteria’s infectionsShow MoreRelatedReview of Related Literature1643 Words   |  7 PagesReview of Related Literature The review of related literatures of this study is made here in the Philippines and in abroad. It is acquired from the internet and unpublished theses. It is nearly related on the relationship of computer game addiction to interpersonal relationship to adolescents especially, K-12 students. To enrich the background on the subject of the researcher, the literatures that were found are here in presented. A. Foreign Studies Based from the study of Mark Griffiths (2007)Read MoreReview of Related Literature668 Words   |  3 PagesReview of Related Literature This section deals with the literature and reading related to the variable of the study. This section also aims to show previous studies of money management which served as the fundamental assumption for the methodology used in the study. Sources of information were taken from the internet, books, journals, magazines, and newspapers. The money management and financial capabilities of CBAE students in the University of Mindanao reading helps the researchers in seeing theRead MoreReview of Related Literature3694 Words   |  15 Pageshuman diseases like malaria, filariasis, Japanese encephalitis, dengue haemorrhagic fever and yellow fever causing millions of deaths every year [1]. Extensive use of chemical insecticides for control of vector borne diseases has created problems related to physiological resistance to vectors, adverse environmental effects, high operational cost and community acceptance [2]. Numerous plant products have been reported either as insecticides for killing lar vae or adult mosquitoes or as repellents forRead MoreReview of Related Literature and Studies1403 Words   |  6 PagesCHAPTER 2 REVIEW OF RELATED LITERATURE AND STUDIES Studies rely on information researched by the proponents. Review of Related Literature In exploration, we find new techniques, new knowledge, even develop new substances, gadgets, equipment, processes or procedures, imagination and skill is employed by the researcher. The commodities, new devices, services, in technology are needs of man for a better fuller life which is the concern of the research. These useful arts are the products of the technologicalRead MoreReview Of Related Literature And Studies1438 Words   |  6 PagesChapter 2 Review of Related Literature and Studies This part of the study contains related literature and studies after the thorough and in-depth research of the researcher. Related Literatures come from both Foreign and Local published literatures that became the basis for the researcher to pursue this study. Related Studies come from both Foreign and Local Studies that has relationship or in relation on her study. Foreign Literature Culture-shaping is a methodological and comprehensive approachRead MoreReview Of Related Literature And Studies Essay1585 Words   |  7 PagesCHAPTER 2 Review of Related Literature and Studies This part of the study contains related literature and studies after the thorough and in-depth research of the researcher. Related literatures come from both foreign and local published literatures that became the basis for the researcher to pursue this study. Related studies come from both foreign and local studies that has relationship or in relation on her study. Foreign Literature Culture-shaping is a methodological and comprehensive approachRead MoreReview of Related Literature and Studies1069 Words   |  5 PagesCHAPTER II REVIEW OF RELATED LITERATURE AND STUDIES LOCAL LITERATURE Josefina Estolas in the book Fundamentals of Research (1995). In exploration, we find new techniques, new knowledge, even develop new substances, gadgets, equipment, processes or procedures, imagination and skill is employed by the researcher. The commodities, new devices, services, in technology are needs of man for a better fuller life which is the concern of the research. These useful arts are the products of the technologicalRead MoreReview of Related Foreign Literature1844 Words   |  8 PagesREVIEW OF RELATED LITERATURE AND STUDIES This chapter presents the review of related literature and studies. It contains a review of books and studies on the subject matter conducted here and abroad and their relation to the present research. The researcher looked for a number of foreign and local studies and some related literatures that have either direct or indirect bearing to the study. Foreign Literature Student Retention Policy Department of Special Education, Counseling, and Student AffairsRead MoreReview of Related Literature and Studies3079 Words   |  13 PagesREVIEW OF RELATED LITERATURE AND STUDIES Several studies have been conducted here and abroad which have a direct and indirect bearing on the subject to be investigated in this study. A brief review of the related literature and studies are presented hereunder. Literature In the present educational system, studies based on the achievement levels of elementary and secondary students revealed that the equality of education has deteriorated over the years. In a recent UNESCO statistics Barsaga (2000)Read MoreReview Of Related Literature And Studies1921 Words   |  8 PagesCHAPTER II REVIEW OF RELATED LITERATURE AND STUDIES Various publications and studies related to the topic being studied were read and analyzed by the researcher to support the claims presented by this study. 2.1 Mobile Robot According to Al-Sahib (2015), the term robot is derived from the Czech word â€Å"robota† which means forced labor. Goris (2004) also said that a robot can be defined as a device that can execute automated tasks, either with the influence of direct human control, or a set of general

Military Pension Reform Military Reform - 1583 Words

Military Pension Reform Military pension reform is a subject that strikes fear in the hearts of many of those serving in the United States military and something which would make me cringe when I heard rumors of it. The current military pension system gives those service members retiring with at least 20 years of service, a monthly entitlement of 50% of their base pay for the rest of their lives. This means that someone who enlists in the military at 18 years of age can begin collecting their retirement at age 38. This percentage can also go up if the member gains enough rank to stay in the military past the 20 year mark. This is an incredible benefit, considering the full social security retirement age is now 65, and soon rising to 67. Reservists collect their retirement at age 60. For the Active Duty member, this equates to 27 years of additional income for the military retiree. The Military Compensation and Retirement Modernization Commission (MCRMC) are working to change this benefit from its cur rent status, to a blended system. The main issue with the current military pension system is an unsustainable cost to the government. While the current military pension system is great for our service members, it is costing the government too much money. â€Å"Military pay and benefits — including retirement pay for veterans — eat up about one-third of the Defense Department budget† (Harper, 2015, para. 2). As illustrated by Harper (2015) the current system cost more thanShow MoreRelatedPatrick Henrys Impact On Slavery1708 Words   |  7 Pagesthey wanted so badly. One of the ways in which blacks were awarded new opportunities was in the military. During the American Revolution, there were two sides of the war; the Loyalists who were loyal to King George III and the British government, and the Patriots who supported Thomas Jefferson’s Declaration of Independence. 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After all, both countries experienced socioeconomic development, the rise of the industrial working class, and the rise of new liberal values. Another puzzling

The Secret of Ella and Micha Chapter 4 Free Essays

Micha â€Å"She’s already got you all hot and bothered.† Ethan sips on his soda. â€Å"Look at you. We will write a custom essay sample on The Secret of Ella and Micha Chapter 4 or any similar topic only for you Order Now Drunk after eight months of sobriety and I don’t believe it’s a coincidence it happened on the same night she showed up.† I slam another shot back and wipe my lips with the back of my hand. â€Å"I’m fine man. And I can’t blame what I do on anyone else but myself. This isn’t Ella’s fault. â€Å" Ethan laughs, tipping his head back, bumping it on the edge of the cupboard. â€Å"Who the hell are you trying to convince? You know just as well as every single person in the room knows that you two are each other’s problem and it’s never going to be fixed until you fuck and get it over with.† I punch him in the arm, harder than I planned. â€Å"Watch it. You’re walking on thin ice tonight.† He holds up his hands, surrendering. â€Å"Sorry, I forgot how you get when you’re like this.† I grab a fist full of his shirt and jerk him toward me. â€Å"Like what?† Again, he forfeits up his hands. â€Å"Micha man, calm down and go drink some coffee or something. You’re trashed out of your mind.† I release him and rake my fingers through my hair, frustrated with something I can’t grasp. â€Å"Coffee’s a myth†¦ And I need something else.† My eyes travel to the back door window, and suddenly I understand what I need. I pat Ethan’s shoulder. â€Å"Clear everyone out before my mom gets home, okay?† â€Å"Alright, man will do,† he replies confoundedly. â€Å"But where are you going?† â€Å"On a walk.† I knock people out of my way, and stumble out the back door. Regaining my balance, I trip across the grass and climb over the fence. Ella’s dad’s Firebird is parked in the driveway, so he must be home from the bar. Doesn’t matter, though. He won’t notice or care if I sneak in. I’ve been doing it since we were kids. Although, my intentions did get a bit dirtier the older we got. I stare up at her bedroom window until I reach the tree. After a drunken struggle, I make it to the top and I inch along the branch to the window. Cupping me hands around my eyes, I peek inside. The lights are off, but the glow of the moon lights a trail to her bed. She’s fast asleep. I inch open the window, slicing my finger on a rusty nail. â€Å"Mother†¦Ã¢â‚¬  I suck on my finger tip, the taste of blood and vodka bitter against my tongue as I head dive through the window and hit the floor with a soft thud. Her friend shoots upright from the bed on the floor, her eyes wide. â€Å"Oh my God.† I put my finger to my lips as I get to my feet. â€Å"Shh†¦Ã¢â‚¬  She still looks worried so I dazzle her with my most charming smile. That seems to win her over and she settles back in her bed. As carefully as I can, I step over her bed and crawl in with Ella. She’s always been a heavy sleeper and doesn’t stir. I press my chest against her back, drape my arm over her waist, and feel the rhythm of her breathing. God, I’ve missed this way too much. It’s not healthy. I burrow my face in her neck, smelling the scent of her hair, vanilla mixed with something that’s only her. I shut my eyes and for the first time in eight months, I fall into a peaceful sleep. Ella I sleep horribly for half the night, tossing and turning, like the princess sleeping on a pea. Only I am far from a princess and the pea is my guilty conscience. I don’t know why I feel guilty about blowing off Micha. I’ve done it breezily for the past eight months. Although, he wasn’t living right next door with his sad puppy dog eyes and charming sexiness. My sleep deprivation only got worse when my dad stumbled into the house in the middle of the night, bumping over cups and bottles, drunk off his ass. Later, I heard him crying in the bathroom my mom died in. It still hurts to hear because his tears are my fault. Once I fall asleep, I am out and it ends up being the best night’s rest I’ve had in ages. When I wake up in the late afternoon, I feel refreshed and calm. Until I realize why. Micha is in my bed and has me in his long, lean arms. His body is curved into mine, so every single part of him is touching me. I know it’s him by the smell of his cologne mixed with mint and something else that only belongs to Micha. I pretend to be asleep, engulfed in a wonderful dream, refusing to wake up until he leaves. â€Å"I know you’re awake,† he whispers in my ear. His voice is hoarse and his breath is stale with booze. â€Å"So open your eyes and quit avoiding me.† â€Å"You know it’s illegal to walk into someone’s house without permission,† I say with my eyes shut. â€Å"And sneaking into someone’s bed – that’s the move of a pervert.† â€Å"I didn’t walk in. I fell in,† he says, amused. I pinch his firm chest and he laughs. â€Å"Now there’s my feisty girl.† He brushes his soft lips across my forehead. â€Å"I’ve missed you, Ella May.† Opening my eyes, I wiggle in his arms. â€Å"Please don’t start. It’s too early.† His eyes are guarded and his hair is a mess. He chuckles lowly, a sound that ripples deep inside my core. â€Å"Pretend all you want, pretty girl. You and I know that deep down you’re secretly glad to be pressed up to my body.† He urges our chests together as he snakes his legs around mine. My eyelids flutter against his warmth. God, I’ve missed this so much. Way too much and so has my body, evidently. â€Å"So where did you go?† he asks, crushing my moment of bliss. â€Å"To school in Vegas? Because it kind of surprises me. You never really liked school.† My mind snaps back to reality. â€Å"I don’t want to get into this right now. I just want to have a relaxing summer and then I’m headed back to campus.† He blinks, his eyelashes fluttering against my forehead. The feel of him sends a warm tingle up my thighs and I seal my lips to keep from moaning. His eyebrows knit. â€Å"It’s like you’ve been kidnapped by a bunch of nuns or something.† â€Å"Maybe I was,† I say submissively. â€Å"It wouldn’t hurt anyone if I was.† He considers this and an artful smirk curves at his lip. â€Å"That’s not true. Nuns can’t have sex and I still haven’t fulfilled my lifelong dream of having sex with you.† I open my mouth, my tongue locked and loaded with an equally perverted comeback, but I bite down, remembering I’m not that kind of a girl anymore. â€Å"I need to wake Lila up. She’s got a long drive ahead of her.† With one swift roll, he has me pinned down beneath his body and my arms trapped above my head. His aqua eyes search mine and it’s like staring at the endless ocean. He sucks on his lip ring, lost in thought. â€Å"You’re going to tell me, pretty girl,† he asserts, tilting his head so his lips are next to my cheek. â€Å"You always tell me everything.† â€Å"Micha, please†¦.† I despise how breathless I sound. â€Å"You know why I left. You were there that night†¦ you saw me†¦ I can’t do it again.† Anxiety claws up my throat and my muscles tense beneath the weight of his body. â€Å"Please let me up. I can’t breathe.† He props up on his arms. â€Å"You could have talked to me, instead of running away. You know that.† I shake my head. â€Å"No, I couldn’t. Not that time. That time it was different. You were part of the reason I had to leave.† â€Å"Because you kissed me?† he asks, dipping his voice to a husky growl. â€Å"Or because I found you that way†¦ that night.† I swallow the giant lump in my throat. The kiss was part of it. It was an earthshattering kiss, one that stole breaths, stopped hearts, and scared the shit out of me because it surfaced feelings I’d never felt before, ones that rendered me helpless. â€Å"I don’t want to talk about it. Now get off of me.† I wiggle my arms between us and push on his chest. He sighs and rolls off me. â€Å"Fine, don’t talk about it, but it doesn’t mean you can run away from me again. I’ll chase you down this time,† he threatens with a wink as he climbs off the bed, and the chain hooked to his studded belt jingles. â€Å"Get dressed and meet me out in the driveway. You have to go visit Grady today.† â€Å"No, thanks,† I decline and tug the blanket over my head. â€Å"And I told you last night I have stuff to do today. Besides aren’t you hung over from last night? You were pretty wasted.† â€Å"Don’t do that,† he says, aggravated. â€Å"Don’t pretend like you have some deep insight into me anymore. You’ve been gone for eight months and a lot has changed.† I’m speechless. â€Å"Micha, I†¦ â€Å" â€Å"Come on, get out of bed. You’re going to see Grady, whether you like it or not.† He yanks the blanket off me and tosses it on the floor, so I’m lying there in my plaid shorts and skin-tight tank top with no bra on underneath. He gives me a prolonged once over, with a dark, lustful glint in his eyes and goosebumps sprout all over my skin. I cover myself with my arms. â€Å"I’m not going to Grady’s. I just got home and I have things to do.† â€Å"He’s got cancer, Ella.† He backs for the door, tucking his hands into the pockets of his faded jeans. â€Å"So get your bratty, split-personality ass out of bed and go see him before you can’t.† My arms fall to my sides as I sit up. â€Å"Why didn’t anyone tell me?† â€Å"If you would have told someone where you were, we would have,† he says. â€Å"Although, I’m pretty sure your father knew where you were, he just wouldn’t tell anyone.† I don’t deny it. â€Å"Besides, I told you in the voicemail I left yesterday,† he says, glancing at my phone on the desk. â€Å"But I’m guessing you haven’t listened to that?† I shake my head. â€Å"No, I was too surprised to see your number on the screen.† He bites on his lip ring, something he does when he’s nervous. â€Å"Yeah, you should probably just delete that. I don’t think you’re ready for it yet.† My gaze moves to my phone. What the hell is on it? I climb out of bed, arching my back and stretching like a cat. â€Å"How bad is Grady?† He swallows hard. â€Å"He’s dying, so you need to get dressed and let me take you to see him.† I begin to object, but rethink my initial stupidity. Grady is the one part of my past that I could never run from. At one point, he was like a father to Micha and me. I even called him from Vegas once, although I didn’t tell him where I was. I nod. â€Å"Let me get dressed and I’ll be out in a second.† â€Å"See you in a few.† He winks at me and vanishes into the hall, leaving the door wide open behind him. Lila quickly springs up from the trundle bed, clutching the sheet. â€Å"Oh. My. Hell. What was that about? I mean, he crawled in here through the window in the middle of the night, and just climbed into bed with you.† â€Å"That’s what he does.† I open the window letting in the gentle breeze. Loose pieces of my hair dance around the frame of my face. â€Å"Oh, no.† Lila stretches her arms above her head. â€Å"What’s wrong?† I reluctantly look at her. â€Å"I think someone might have confused your car for a canvas.† She jumps out of bed and elbows me out of the way to get a look at the damage done to her beautiful, nearly brand new Mercedes. â€Å"My poor baby!† I pull a skirt and a pink tank top out of my duffel bag. â€Å"Get dressed and we’ll go check out the damage.† She pouts, looking like she might cry. â€Å"I can’t drive it home like that. My parents will kill me.† â€Å"I know plenty of people who can fix it for you,† I say, opening the door. â€Å"Or I use to, but I’m sure it’s all the same.† She nods and I go to the downstairs bathroom to change, avoiding the upstairs one. I turn on the shower so the mirror will fog up and hide my reflection. I comb my hair until it flips up at the ends naturally. Then I apply a light shade of lip gloss and head out the door, but run into my dad on the stairway. â€Å"When did you get here?† His breath smells like gin and his eyes are red. His cheeks have sunken in over the last eight months and his skin is wrinkled like leather with sores. He’s in his late forties, but looks like he’s pushing sixty. â€Å"Last night,† I tell him, taking his arm and helping him up the stairs. â€Å"I was in bed before you got home.† He offers me a pat on the back. â€Å"Well, I’m glad to have you home.† â€Å"I’m glad to be home,† I lie with a smile as we reach the top of the stairs. He moves his arm away from my hand and rubs the back of his neck. â€Å"Do you need anything? Like help carrying in your boxes?† â€Å"I think I can handle it on my own, but thanks.† I decline, sticking my arm out as he teeters toward the stairs. He nods and his eyes drift to the bathroom down the hall. He’s probably thinking about how much I look like her. It hurts his eyes, at least that’s what he told me the night I went to the bridge. â€Å"I guess I’ll talk to you later then. Maybe we could go to dinner or something?† He doesn’t leave me time to answer as he zigzags down the hall to his room, slamming the door shut behind him. My dad started drinking when I was about six, a few months after my mom got diagnosed with a bipolar disorder. His drinking habit wasn’t that bad back then. He would spend a few nights at the bar and sometimes on the weekends, but after my mom died, beer and vodka took over both our lives. When I return to my room, Lila is dressed in a yellow sundress, with her blonde hair curled up and there is a pair of overly large sunglasses concealing her eyes. â€Å"I feel like crap,† she declares, putting her hands on her hips. â€Å"This place has that effect on most people.† I grab my phone, noting the flashing voicemail as I slip on my flip flops. We go outside, leaving the smoky air behind and step into the bright sunlight, surrounded by the scenery of rundown homes and apartments. The neighborhood is filled with motorcycle engines revving and far in the distance are the sounds of a lovers’ quarrel and Micha is nowhere to be seen. A long time ago, it felt like home, back when street racing and running wild felt natural, but now I just feel lost. Lila starts biting at her fingernails as she gapes confoundedly at her car. â€Å"It looks worse up close.† I circle her car with my arms folded, assessing the damage. It looks like a fruit basket, only instead of being filled with fruit it’s crammed with innuendos and colorful words. I’m on the verge of laughing for some reason. â€Å"They got you good.† She shakes her head. â€Å"This isn’t funny. Do you know how much it’s going to cost to fix this?† Lila’s dad is a big shot lawyer over in California. Her parents are always sending her things like clothes, money, cars. She has never worked a day in her life and gave me a hard time for my waitressing job at Applebee’s, begging me to take time off to go to parties. â€Å"So what do we do?† She chips at some green paint on the headlight with her fingernail. I point up the street. â€Å"There’s an auto body shop not too far from here.† She glances down the road, which is covered in potholes and lined with filthy gutters. â€Å"But this is a Mercedes.† â€Å"I’m sure painting a car, no matter who the maker is, is all the same.† â€Å"But what if they do something to it?† â€Å"Like spray paint it again after they paint it?† I say sarcastically and she scowls. â€Å"Sorry. We’ll find someone, okay? We can take it to someplace in Alpine. It’s a little nicer over there.† â€Å"I can’t drive it when it looks like this,† she complains, motioning at the car. â€Å"It’s hideous.† â€Å"I’ll drive it, then,† I offer my hand out for her to give me the keys. â€Å"Are you joking?† She pats the hood of her car. â€Å"This is my baby. No one drives it but me. You know that.† â€Å"I think your baby is in serious need of some plastic surgery.† Micha strides off the porch of his house and onto the driveway. He’s changed into black jeans, a fitted grey t-shirt, and his blonde hair hangs in his eyes. Using his long legs, he jumps over the chain-linked fence between our yards. â€Å"I know the perfect place to get it fixed and it’s here in town, so you won’t have to drive it so far.† He gives Lila a wink. â€Å"I’m Micha, by the way.† â€Å"Hi, I’m Ella’s roommate or old roommate anyway,† she says with a warm smile and slides her sunglasses down the brim of her nose. â€Å"We’re not sure if we’re sharing a dorm room next semester.† He presents her with his player grin. â€Å"Sharing a room with Ella? That had to be tough.† He shoots me a mischievous look, trying to get a rise out of me. She laughs and returns her glasses over her eyes. â€Å"No, she’s a pretty great roommate, actually. She cleans and cooks and everything. It’s like having my own house maid.† â€Å"Ella was always good at that stuff,† he agrees, knowing the real reason why. Even before my mom died, she was never good at taking care of the house. I had to learn how to take care of myself at a very young age, otherwise I’d have starved and rotted away in a rat-infested house. â€Å"So do you want me to take your car to that shop I was talking about? Like I said, it’s really close.† â€Å"Yeah, that sounds great.† She shuffles her sandals against the concrete. â€Å"I’d rather go someplace close.† I mentally roll my eyes. Leave it to Micha. He can get any woman to contradict herself if he wants to. He swings his arm around my shoulder and kisses me on the forehead. â€Å"But I have to take pretty girl over here to see an old friend first.† â€Å"Please stop calling me that,† I beg. â€Å"I’ve never liked the nickname and you know that. I never even got why you called me it.† â€Å"And that’s the appeal of it, pretty girl.† He tempts me closer to him and caresses my cheek with his lips, giving me a kiss that brings warmth to my skin. â€Å"Now are you ready to go see Grady? You can come, too, if you want†¦ is it Lila?† â€Å"Yeah, it’s Lila. Lila Summers.† She offers her hand and Micha shakes it. â€Å"And sure I’ll go. This place makes me a little nervous.† â€Å"Isn’t your family expecting you to be home tonight?† I escape from underneath Micha’s arm. â€Å"I’ll text them and tell them I’m not leaving until tomorrow.† She retrieves her cell phone and scrolls through her contacts. â€Å"The car will be done by tomorrow, right?† â€Å"It’s hard to say,† Micha says. â€Å"Ethan is the best, but a little slow.† Her head snaps up and there’s delight in her eyes. â€Å"Ethan as in Ethan from the party last night? The one with the sexy hair and the really big hands?† Micha bites down on his lip, stifling a laugh, and flicks me a sideways glance. I can’t help but smile. â€Å"Yep, that’s the one,† he says. â€Å"Do you feel better about taking your car to him now?† â€Å"Well, yeah, if you think it’s okay?† she checks. â€Å"I’m very picky about who works on my car or at least my dad is very picky about who works on it.† â€Å"It’ll be fine,† he assures her with a wink. â€Å"I’ve never disappointed a girl yet.† â€Å"Oh yeah?† Lila laughs, glancing at me uneasily, like she’s worried she’s stepping on my territory. â€Å"So are we going to go or what?† A ping of jealousy pinches inside my chest. â€Å"Yep, let’s go, beautiful.† Micha leads the way around the fence and up his driveway to the garage. When I step inside, my mouth falls open. Parked in the middle, between the walls lined with shelves and tools, is a shiny 1969 Chevy Chevelle SS. It’s painted in a smoky black with a cherry red racing stripe down the center. â€Å"You finally fixed it up?† He pats the shiny flawless hood, his eyes sparkling with excitement. â€Å"I finally got around to it, after talking about it for four years.† His eyes find mine, seeking my approval. â€Å"So what do you think?† â€Å"It’s kind of old.† Lila pulls a face at the car. â€Å"And really big.† â€Å"I thought you liked things big?† Micha teases. I punch him in the arm and he laughs. â€Å"Ow, I meant hands. Jeez get your mind out of the gutter.† I roll my eyes. â€Å"You did not, you pervert.† He shrugs, his eyes lustrous in the sunlight filtering through the gritty windows. â€Å"So what. It got you to lighten up, didn’t it?† â€Å"Are we driving it to Grady’s?† I opt for a neutral voice. He slips his keys out of his pocket and tosses them to me. â€Å"Yeah, go ahead. It’s all yours.† I swiftly shake my head and throw the keys back at him, like they’re scorching hot. â€Å"No thanks. I don’t want to.† He cocks an eyebrow, looking sexy. â€Å"What do you mean you don’t want to?† â€Å"I mean I don’t want to drive it.† It nearly kills me to say it. I walk around the front of the car, open the door, and gesture for Lila to get in. â€Å"But it has a blown 572 Big Block in it,† he says astounded with the keys hanging loosely from his fingers. â€Å"How can you not want to drive it?† My insides twitch to drive it, but I won’t buckle. â€Å"It’s fine, Micha. I’d rather be the passenger.† â€Å"What does that mean? A blown big block or whatever you said?† Lila wonders as she walks to the side of the car. â€Å"Wait, are you guys talking cars? El doesn’t like cars. In fact, she made us take the bus most of the time when we left campus.† â€Å"Oh really?† His tone implies otherwise. â€Å"That’s news to me.† â€Å"It’s a waste of gas,† I lie, attempting to mask the truth; that I miss it. The rush, the speed, the adrenaline high. Lila ducks inside the car and into the backseat. I climb into the passenger side and Micha opens the garage door. He revs up the engine, letting it rumble, teasing me, before backing down the driveway. â€Å"I’m starting to think that the Ella you knew isn’t the same one as I know.† Lila buckles her seatbelt up. He spins the tires down the road. â€Å"I think you might be on to something Lila, because the one I knew loved cars. In fact she used to hang out in the garage all day with the guys while the other girls played with their hair and makeup.† He flashes me a dangerous grin. â€Å"She used to get all excited when we’d go racing.† No matter how hard I try to hinder myself from getting energized, I can’t. Those hot summer nights, flying down the highway, neck and neck with another car, the rush soaring through my body. Micha trails his finger along my neck and rests it on my pulse. â€Å"You’re getting excited just thinking about it.† His touch spreads a longing through my body. I swat his hand away, cross my arms, and focus on the window, watching the neighborhood blur by as he cruises over the speed limit. Micha shifts the gears and the engine thunders louder, begging to burn rubber. â€Å"Is it legal to be driving this fast?† Lila asks nervously. We glance back at her and she grips the edge of the leather seat. â€Å"It just seems like we’re going really fast, especially in a neighborhood.† Micha holds my gaze resolutely as he downshifts and pumps up the rpms. â€Å"What do you think? Speed up? Or slow down?† I want to tell him to slow down, pull on my seatbelt, and look away, but a passion that was dead raves. He throttles the gas pedal, keeping his eyes on mine, venturing me to look away first. â€Å"Um†¦ I don’t think this is a good idea.† Lila’s voice is far away. The car surges faster down the narrow road and his eyes dare me to tell him to slow down and part of me wants to. Desperately. But as he shoves the shifter into the next gear, going faster and faster, my body pleads to let go. Suddenly, Lila screams, â€Å"Stop sign!† Micha’s eyes sparkle like sunlight reflecting into the ocean. He slams on the brakes, squealing the car to a halt, and throwing us all forward. My hand shoots out and I brace myself from hitting the dashboard. â€Å"Are you crazy?† Lila’s voice cracks as she situates back into the seat and realigns her dress over her legs. â€Å"What is wrong with you two?† Micha and I look at each other and my body is burning with a hidden desire that I won’t admit exists. My heart beats in my chest, rock steady and alive again. For a second, I’m back in the place I lost. Then Micha ruins it. â€Å"See, the same old Ella still lives.† He grins arrogantly as he drives through the intersection. â€Å"She just needed a little push out.† I click the seatbelt locked, proving a point. â€Å"No, she doesn’t. She’s gone forever.† â€Å"Try all you want, but I’m bringing her back.† He bites his lip, refocusing on the street as he mutters, â€Å"I won’t let that night ruin you forever.† But it did. It broke me into a million pieces and blew them away in the wind, like crumbled leaves. That night was one of the most incredible nights I’ve ever had. Then I quickly plummeted toward rock bottom. How to cite The Secret of Ella and Micha Chapter 4, Essay examples

End of Life Care free essay sample

It was recognised by Jevon (2009) that caring for the dying patient can be the most fulfilling whilst also most challenging times for nurses and healthcare professionals. This paper will introduce a case study based on personal experience within clinical placement, exploring and analysing the above statement. In accordance with the Nursing and Midwifery Council Guidelines (2008) names have been changed to protect patient confidentiality. The paper will seek to address issues relating to the case study examining relevant theory. Current policies and guidelines will be considered in relation to the patient. It will identify a framework involved looking at literature to assess the effectiveness against the case study. Finally, focusing on a specific area of care, which in this case will be communication, the paper will evaluate the care given within the case study before providing a conclusion. Mrs Jones, a 65 year old lady diagnosed with lung cancer in July 2010, lived with her husband of 29 years. They had two grown up daughters that lived close by their home. When Mrs Jones first received the diagnosis by her consultant her family were present. After discussions it was decided that the best care option for them was to care for Mrs Jones within their home. More investigative tests revealed that the cancer had spread; with this her condition seemed to deteriorate. Although appreciative of the support they were receiving within their home, Mr Jones and his daughters were finding it difficult to control Mrs Jones symptoms and pain management. Following a family discussion enquiries were made regarding the possibility of hospice care. Hospice care has been described as providing humane and compassionate care for people in the last phase of incurable disease (American Cancer Society, 2013). Within two weeks Mrs Jones was admitted into their local hospice. Symptom and pain management issues were addressed and Mrs Jones seemed relaxed and free from pain and discomfort. Mr Jones and her daughters visited frequently, towards her final days they decided to reside in the family room within the hospice. When it was no longer possible for Mrs Jones to consume food or liquids a decision was reached by the multi-disciplinary network within the hospice to commence the Liverpool Care Pathway. The hospice staff took the time to sit with the family and explain how the pathway works allowing time for any questions or objections. Within 48 hours Mrs Jones died surrounded by her family. ‘We cannot take away the whole hard thing that is happening, but we can help to ring the burden to manageable proportions’ (Cicely Saunders, as cited inEllershaw amp; Wilkinson, 2003) this was a comment made during a seminar in 1962 and is thought to be an early attempt at challenging the way in which health professionals approach end of life care. End of life care has been defined by the National Council for Palliative Care (2006) as helping ‘†¦all those with advanced, progressive or incurable illness to live as well as possible until they die. It enables the supportive and palliative care needs of both patient and family to be identified and met throughout the last phase of life and into bereavement. It includes management of pain and other symptoms and provision of psychological, social, spiritual and practical support. ’ Dame Cicely Saunders, founder of St Christopher Hospice in 1967 paved the way and had a major impact on changing people’s attitude and thinking regarding end of life care. Primarily a small sample of only four patients was chosen to experiment with drug dosages. This was to assess the impact of regular and consistent administration of medications. The results of this study indicated an improvement in quality of life. Consequently, since the work of Dame Cicely, end of life care has advanced, further studies undertaken by Professor John Hinton established a link between physical and mental distress of patients at end of life care. Professor Ann Cartwright was responsible for a large-scale epidemiological study through surveys to a random sample of patients and families in end of life care. This was a comparative study between 785 subjects in 1969 and 639 subjects in 1987. Results revealed an increase in the amount of people living longer with prolonged illness, greater awareness of death and dying alone within acute hospital settings. However, it did highlight an improvement in home help although, fewer visits where occurring (Cartwright, 1991). Referring back to Mrs Jones, the latter of the findings was evident. Although Mr Jones was appreciative for support being received within the home and community environment, he was having difficulties controlling his wife’s symptoms and pain management between health professionals visits. An implication of this is the possibility that Mrs Jones preferred place of care was compromised resulting in re-evaluation of the family’s situation, therefore, potentially meaning that Mrs Jones wishes were not met. It is from studies such as the ones stated above that progression in end of life care has evolved with the introduction of palliative care nurses such as Macmillan nurses and an increased number of hospice’s being provided worldwide. The main focus at end of life care was predominantly based on cancer patients. Initiatives such as the Comprehensive Cancer Care Programme was initiated in 1980 focusing on areas including prevention of illness, early detection, treatment and pain relief and palliative care. This programme was introduced by the World Health Organisation it is reported by Twycross (2007-2008) as prompting other associations and initiatives at global and national levels. However, after extensive work and research it was only in 2008 that a strategy was devised by the Department of Health, which aims to promote high quality care for patients receiving end of life care by giving patients the option to choose where they would like to receive end of life care (Department of Health, 2008). Nevertheless, one question that needs to be asked, is whether enough has been applied to healthcare services and nursing to manage and support patients and families in end of life care? The modern hospice movement was mainly devised from a Christian tradition, hospices are now well established within end of life care. Hospices began by being mainly focused on cancer patients altough better knowledge and understanding of other incurable desease has seen an increase in patients admitted with other conditions. An end of life care programme was formulated, consisting of six steps, within the end of life care strategy. It was recognised by the Department of Health (2008) that care pathways are useful tools within health care. Care pathways have been defined as anticipated care placed in an appropriate time frame, written and agreed by a multi-disciplinary team (Davis, 2005) it is believed that care pathways determine multi-disciplinary working by using evidence and guidelines for certain patient groups (Overill, 1998) however Kinsman, Rotter amp; Snow (2001) state that a definition is unclear and no agreement has been made. Considering the six steps of the end of life care pathway the paper will examine Mrs Jones situation. The pathway begins with discussions regarding diagnosis and recognising a patient needing end of life care support. Fallowfield, Jenkins amp; Beveridge (2002) suggest that health professionals try to protect themselves from breaking bad news to patients. This was not evident with Mrs Jones as her consultant was honest and informative when breaking the news of her diagnosis. Although, it still highlights the importance of communication, especially in the initial diagnosis as the patient deserves to have clear and factual knowledge about their prognosis. It was recognised by Watts (2009) that communictaion is a key component in end of life care, specifically in regards to psychosocial and spirtual care. Inadequate communication could prove detrimental to the care plans developed at a later stage. Step two relates to the assessment, care planning and review of care. This is where advance care planning is introduced to the patient and family. An advanced care plan allows the patient to express their wishes on care and treatment options, which can be recorded by health professionals and used when mental capacity is lost. It is believed that advanced care planning is a thoughtful process which allows for patient values and beliefs to be considered within their end of life care pathway (Schaffer, 2009). Mrs Jones discussed an advanced care plan and whilst it did not affect the outcome or care received the implications had this not have discussed could have been distressing for the family. Lynn, Schuster, amp; Wilkinson (2007) believe that without an advanced care plan a crisis situation could occur, causing conflict between family and health professionals. There could be many reasons why patient’s decline discussions on advanced care planning one of which being that they do not understand. When an advanced care plan has been recorded within patient records and transferred between health organisations coordination of care can be acheived. As the third step in the end of life care programme it ensures that patient preferences are being adhered to. The important component within this step is communication and multidisciplinary and mulitiagency working. Delivery of high quality care is covered by step four in the end of life care programme, referring back to Mrs Jones it could be argued that when being cared for within her own home high quality care was not being delivered consistently as her symptoms and pain management were not controlled adequately. A study conducted by OBrien (2010)found that poor discharge planning, co-ordination, difficulty in establishing additional quipment along with inadequate out of hours services resulted in patients care preferences not being adhered to. As was the case for Mrs Jones who was admitted into her local hospice. It is stated in the end of life programme that the same high quality of care should be received regardless of which setting. Within the hospice Mrs Jones needs were met upon arrival with analgesia which as Dame Cicely’s study revealed made an impact on a patient’s q uality of life in end of care provisions. It was recognised when Mrs Jones entered into the final phase of life, at which time the hospice staff spoke to Mr Jones and his daughters to explain about the Liverpool Care Pathway. The Liverpool Care Pathway was devised as a nurse led document to empower and provide a clear action plan when caring for patients during their final phase of life. Although the Liverpool Care Pathway is still relatively new to the National Health service, there has been much controversy due to bad media coverage. Devlin (2009) reported that a group of experts claimed patients were being wrongly judged as close to death. What media fails to report are cases where patients benefit from hospice levels of care ensuring that the patient is comfortable, without pain and having the ability to nurses to administer medications as soon as they are required without delays whilst waiting for medical staff. Once the pathway had been explained to Mrs Jones family and they was happy to proceed with the pathway it was commenced. The final step in the end of life care programme considers care after death. Last offices are performed after death when procedures are followed to prepare for transferal of the body to a chapel of rest, mortuary or undertakers (Jevon, 2009). It is important to consider the families religious and cultural beliefs when undertaking this final step as this stage focuses upon showing respect for the deceased whilst fullfilling requirements (Dougherty amp; Lister, 2004). Mrs Jones did not express any cultural or religious considerations and so a standard last offices was performed. Following last offices Mr Jones and his daughters viewed Mrs Jones body to pay their last respects and say their goodbyes. Altschuler (2004) recognises that end of life care and medical diagnosis sometimes leads to changes not only for the individual but also for the amily. Within this section of the paper, considering Mrs Jones and her family, support through end of life care will be explored. Before beginning it is important to respect that not all families function in the same way. A study by Campbell (1996) found that effective support by palliative care teams revealed more satisfied families of patients in end of life care. Hudson, Quinn, OHanl on, amp; Aranda (2008) state that communication between family, patient and health care professionals is paramount in end of life care and also integral to understanding and providing support which is required. Regarding Mrs Jones, at her initial diagnosis it can be said that individually she was well supported, surrounded by her family. Payne, Seymour, amp; Ingleton (2008) state that evidence shows most people do not require additional support as they are resilient and able to adapt to changes with the support of their own networks. It is important not to forget the people that may not have the same support networks in the community. Assumptions regarding support could be detrimental to the overall outcome of the end of life care received and also the aftermath with the family in the bereavement process. When examining literature some contridictory information was found regarding pre-bereavement support for families, whilst Field, Payne, Relf, amp; Reid (2007) found that families benefitted from such services Grande, Farquhar, Barclay, amp; Todd (2004) study indicated less helpful or beneficial results. These could be explained by the difference in expectations, services available and communication techniques. Considering Mrs Jones case study her family accessed support through the staff and services within the hospice. Also before admission into the hospice they were receiving support from MacMillan nurses and other community organisations. The hospice which Mrs Jones was admitted offerred her family bereavement support which includes councellors, and volunteers and the service is available on a self referral after the death of a relative also. Mr Jones declined this service although they did enquire about the rememberance evening held within the hospice. Rememberance evening are described as being mainly non-religious, although, there is a religious section added on for people who require it. Names of recently deceased patients are read aloud with lighting of candles, music, various readings and a chance to share experiences with others who are undergoing the same process of change as yourself(Wigan amp; Leigh Hospice, 2011). From the initial diagnosis Mrs Jones family seemed to support each other through acceptance and grief and therefore felt that it was unnecessary to use the bereavement service. It is evident from writing this paper that end of life care has evolved rapidly and seems to be of major interest at national levels. This will hopefully continue ensuring that better services can be provided in areas such as the community to ensure people like Mrs Jones can be cared for at home receiving the exact levels of treatment as would a patient within a hospice. Until this time advanced care plans can never fully be adhered to as revisions are often required for patients such as Mrs Jones, when symptom and pain management cannot be covered consistently. However it was also shown how hospice care is providing patients and their families with high standards and quality care, through physical and holistic approaches to care. Communication has been highlighted as a key component within end of life care. Overall Mrs Jones received good communication and with this the support systems were accessed as and when required by the family. It is also wise to note that good communication was received by the family regarding the Liverpool Care Pathway. This paper began with a statement by Jevon (2009) in which it was regarded that caring for somebody in their final phase of life can be the most challengin yet most rewarding experience. Further research would help to advance end of life care, making it even more widespread. Upon reflection the care which Mrs Jones received in the community could have been better although that is due to poor co-ordination such as out of hours services. The hospice still seems to provide better levels of care when comparing how Mrs Jones felt in the community compared with how she was in the hospice. Further work needs to be done in order to gather a true reflection on the difference in levels of care between settings. When looking at the end of life care programme it was impressive to see that Mrs Jones was benefitting by receiving the correct care plans. References Altschuler, J. (2004). llness and loss within the family. In P. Firth, amp; G. Luff, Loss, Change and Bereavement in Palliative Care (pp. 53-65). Berkshire: McGraw-Hill Professional Publishing. American Cancer Society. (2013, February). Hospice Care. Retrieved from Hospice Care: http://www. cancer. org/treatment/findingandpayingfortreatment/choosingyourtreatmentteam/hospicecare/index Campbell, M. (1996). Program assessment through outcomes analysis: efficacy of a comprehensive supportive care team for end of life care. AACN Clinical Issues, 156-167. Cartwright, A. (1991). Changes in life and care in the year before death 1969-1987. Journal of Public Health Medicine, 81-87. Davis, N. (2005). Integrated Care Pathways. Retrieved from NHS: http://www. wales. nhs. uk/sitesplus/Documents/829/integratedcarepathways. pdf Department of Health. (2008, 07 16). Publications policy and guidance. Retrieved from Department of Health: http://www. dh. gov. uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_086277 devlin, K. (2009, September 02). The Telegraph. Retrieved from The Telegraph: http://www. telegraph. co. uk/health/healthnews/6127514/Sentenced-to-death-on-the-NHS. html Dougherty, L. amp;. (2004). The Royal Marsden Hospital Manual of Clinical Nursing Procedures 6th Edition. Oxford: Blackwell Publishing. Ellershaw, J. , amp; Wilkinson, S. (2003). Care of the Dying: A Pathway to Excellence (Second Edition). Oxford: Oxford University Press. Fallowfield, L. J. , Jenkins, V. A. , amp; and Beveridge, H. A. (2002). Truth may hurt but deceit hurts more: Communication in Palliative Care. Palliative medicine, 297-303. Field, D. , Payne, S. , Relf, M. , amp; Reid, D. (2007). An overview of adult bereavement support in the United Kingdom: Issues for policy and practice. Social Science and Medicine, 428-438. Grande, G. , Farquhar, M. , Barclay, S. , amp; Todd, C. (2004). Caregiver bereavement outcome: relationship with hospice at home, satisfaction with care, and home death. Journal of Palliative Care,, 69-77. Hudson, P. , Quinn, K. , OHanlon, B. , amp; Aranda, S. (2008). Family meetings in palliative care: Multidisciplinary clinical practice guidelines. BMC Palliative Care, 7-12. Kinsman, L. , Rotter, T. , E, J. , amp; Snow, P. a. (2001). What is a clinical pathway? Development of a definition to inform the debate. BMC Medicine. Lynn, J. , Schuster, J. , amp; Wilkinson, A. a. (2007). Improving care for the End of Life: A Sourcebook for Health Care Managers and Clinicians (2nd Edition). Cary: Oxford

Healthy Living Report free essay sample

Healthy livingâ€Å" Campaign report 1. Background research to the issue To find the way linked to my group topic and target made us to meet many times and discuss about social issues among students and young people. It made my group to think a lot what topic for campaign would be interesting to us – while working for this, and similarly what would be interesting for other people especially for students in Middlesex University. First of all, everyone in my group pay attention on unhealthy food and ingredients which make daily food able to be fresh for long term and even dangerous. My group decided to start doing campaign â€Å"tricky food† and we all fell into research on dangerous ingredients in popular supermarkets’ food. As we published in our Wiki page â€Å"Unhealthy substitutes used in food production† had to be our campaign stage. (Wiki, ResearchS08G1). From long list of various chemicals and acids which are used in food our group made a decision to focus on Aspartame the most. We will write a custom essay sample on Healthy Living Report or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Aspartame is artificial sweetener which is 200 times sweeter than sugar and commonly used in food industry. In the internet is not difficult to find many articles and discussions about it, some people claim that it is very dangerous to human’s health because of its force to cause cancer, nerve disorders, birth defects and other healthy imbalances. From the other side of view, probably from the business side, some people are ready to prove that aspartame is not so bad, the right amount of it in food is not dangerous and it is cheaper to use in food industry than sugar. However, this sweetener is widely used and found in drinks such as diet coke, energy drinks and even some juice. In the United States aspartame is regulated by FDA – Food and Drug Administration which has set the ADI (acceptable daily intake) for aspartame 50mg/kg and similarly in the European Union – by European Food Safety Authority ADI is 40mg/kg. (American Cancer Society, Learn About Cancer 2011). Well, when the national administration has set the secure quantity of this sweetener it became allowable to use it in food widely. Due to that my group decided that there are no argues with food industry and during our campaign it is hard to change sweeteners usage in nourishment and we made another decision: to change campaign topic and target substantially. Every one of us is surrounded by bright adverts and slogans to be eco-friendly, to eat healthy food, to avoid junk food, to choose food without any preservatives and so on. My group and I also decided to join in those who are providing healthy living style. We started to do another research linked to it, to search what vitamins human need to recover healthy organism after winter and which fruit and vegetables have beneficial vitamins, minerals and antioxidants. Also very important issue that UK is on the leading position in regards to obese citizens in Europe (Wiki, ResearchS08G1). Regarding to New York Times (2010, The World is Fat) in almost half of developed countries, one out of every two people is overweight or obese. In the figure is clearly visible that England was and will be second nation after United States which has overweight, till 2020 it can reach 70 per cent limit! These facts made us to take care for obese issue in United Kingdom. As a united group of different countries: two Lithuanians, Polish and two English we see our target to start informing students about this growing problem by providing healthy eating habits and healthy life style including exercises and view of it. 2. Process of campaign planning Firstly, we had chosen to run the campaign about â€Å"tricky food† and especially aspartame but as I mentioned in the first part of report, during the research we found facts that aspartame is legal sweetener and the right amount of it is not dangerous to human’s health. With our campaign we would be having too less force and evidence in order to avoid this sweetener use in food industry. Regarding to this, we left the idea about tricky food and during more than two meetings we were discussing about many topics which could be interesting and worthy to pay attention. It was not easy part of planning our campaign because we all understood that to find the topic with strong target and serious attitude it is difficult. We mentioned points such as how we could include more people to our team, how we could represent information for them and for our university, should we stay only in university or shall we go further: inform people about issue in our living areas, in the social websites, etc. how we could do our event and what to do so as to attract an attention. We were sure that we will have leaflets with useful information, that we will set up facebook page for our campaign and we will do our event in the university. In our meeting we had not have one  person as a chairperson, we all were equivalent and we all were suggesting our ideas. My idea was to look deeper how we are living, what are ours daily diet, daily routine – are we healthy enough or should we change something in our lives. While suggesting the topic Healthy living I also had an idea to raise the charity fund. Even more, to visit children schools or other meeting places and inform them about our campaign and targets. Unfortun ately, I had not got any assent from my team members for this action. However, I sent some emails to charities such as British Red Cross and Save the Children. I informed them about our campaign and targets, mentioned that we would like to do our campaign as a charity. While giving healthy food (fruit or veg) in university my team could collect some donation for one of the charities and later we could collaborate by doing volunteering jobs or events providing healthy living. I have got an answer from British Red Cross, they were interesting in our action and targets. Unfortunately, I had got that answer too late because we were already doing our campaign on ourselves without any outside organizations. 3. Work as a group From my view at this point I am quite happy while working with my group. As in every team work we faced with some advantages and disadvantages but the final result was nice. The best times and really strong team work were when all group members were attending the meetings. Sure, it had happen only few times, however, those meetings were pretty good. We had never have any argues or complaints, our discussions were always polite and with respect to each other. We always listened to each other’s ideas and discussed them together with giving some agreements or reasoned rejections. Usually our meetings were with three attended group members. Nevertheless, we were able to consider what would be the next step, what research we had to do until next meeting and all jobs or duties were apportion between every team member equally. Even more, if we were having enough spare time after meeting, some of us were going to library to do research together or to write down the minutes into the wiki page. For more questions or advice we were chatting online, by sending emails we arranged for the next meeting. On the other hand, this low attendance made some difficulties to organize all team work, it always takes time to text messages to inform other members about further actions. Because everyone has jobs it was difficult to find right times in order to meet all together, some group members were paying more attention for their jobs than to studies and especially for this campaign. Due to these issues our campaign was small and weak, its background were just two people who pay more attention to every organizing step. 4. Your action I value my action in the group as big part of whole campaign. The background and topic of Healthy living was my idea suggested in the group meeting. I came with the wide idea of it and I had some plans how to reach the main aim. As I mentioned above, I offered to do a campaign as a charity: while providing fruit or vegetables for students we can collect donation for a charity, later collaborate with that organization and hope for getting some support. Personally I always like to communicate with organizations such as charities or other help centres, I have previous experience by searching the support for some events that is why I feel free and confident while asking others to promote my plans. This time I had asked some charities but unfortunately I have not got any help from them. First of all, I think I had to call them straight away and reject sending emails and waiting for answers, second , I think I had to let them know more about my action, about our campaign, purpose and all details so as to make them interesting enough to support us. Finally, if I would be able to have enough force to influence others I would have asked other my group members to help me and to pay more attention on our campaign because I felt too weak to take this huge part alone. Anyway, at the end of March and beginning of April I was communicating with Community Fundraiser from British Red Cross. She replay to my email a bit too late than I was expected but it is always nice to let other communities know about my actions and attitude. I can be brave to say that I was that person in my group who was the most enthusiastic about campaign planning and actions. It was not difficult to take care and organize the event during which we were giving free apples, apple shaped stickers and leaflets with interesting information about fruit. In order to get apples each of us asked local shops to support us by giving some apples. I am living Hendon, so here I found many local shops where I am shopping almost every day and I visited them with my special request. First of all, while talking to the manager of the shop I let him know who I am and what I am doing, later I asked him to support our campaign and in the same way I made him sure that he is making big help not only for my group but also for some students who maybe will start thinking more about healthy life style. Some shops rejected my request but I did not give up and went ahead. Finally, I found one shop near to the Hendon Central Station which was very helpful and polite. People who are working there wished me luck and even know they look at me as at friendly shopper. 5. Supporting other campaigns Our group was working alone without any collaborate with other campaigns from our module. As we all got the topic food almost all campaigns were linked to healthy products. Some of them had chosen specifically one product (energy drink, junk food – hamburgers, etc. ) and they run campaigns in order to change people attitude and inform them how dangerous it is. Differently, we had not chosen any products – we looked into whole lifestyle which includes food, exercises and attitude. I really appreciate other people job, how they did their campaigns, how they had represented their ideas and what targets they had. Only one thing what I might to change could be the way how they did their events. Most of all campaigns were too silent, working only on social websites such as Facebook or Twitter, I would rather do it loudly in the centre of our university. References: Wiki, S08G1 â€Å"Healthy Living†, research 2012 [online], http://mcs1000campaigns. middlesex. wikispaces. net/ResearchS08G1 American Cancer Society, Learn about cancer 2011. [online], http://www. cancer. org/Cancer/CancerCauses/OtherCarcinogens/AtHome/aspartame The New York Times, The World is Fat 2012, [online], http://economix. blogs. nytimes. com/2010/09/23/the-world-is-fat/

When to Use GET and POST in Ajax

When to Use GET and POST in Ajax When you use Ajax (Asynchronous JavaScript and XML) to access the server without reloading the web page, you have two choices on how to pass the information for the request to the server: GET or POST. These are the same two options that you have when passing requests to the server to load a new page, but with two differences. The first is that you are only requesting a small piece of information instead of an entire web page. The second and most noticeable difference is that since the Ajax request doesnt appear in the address bar, your visitors wont notice a difference when the request is made. Calls made using GET will not expose the fields and their values anywhere that using POST does not also expose when the call is made from Ajax. What You Should Not Do So, how should we make the choice as to which of these two alternatives should be used? A mistake that some beginners might make is to use GET for most of their calls simply because it is the easier of the two to code. The most noticeable difference between GET and POST calls in Ajax is that GET calls still have the same limit on the amount of data that can be passed as when requesting a new page load. The only difference is that because youre only processing a small amount of data with an Ajax request (or at least thats how you should use it), you are far less likely to run into this length limit from within Ajax like you would with loading a complete web page. A beginner may reserve using POST requests for the few instances where they do need to pass more information that the GET method allows. The best solution when you have lots of data to pass like that is to make multiple Ajax calls passing a few pieces of information at a time. If you are going to pass huge amounts of data all in the one Ajax call, you would probably be better off simply reloading the entire page since there will be no significant difference in the processing time when huge amounts of data are involved. So, if the amount of data to be passed isnt a good reason for choosing between GET and POST, then what should we use to decide? These two methods were in fact set up for entirely different purposes, and the differences between how they work are in part due to the difference in what they are intended to be used for. This not only applies to using GET and POST from Ajax but really anywhere these methods might be employed. The Purpose of GET and POST GET is used as the name implies: to get information. its intended to be used when you are reading information. Browsers will cache the result from a GET request and if the same GET request is made again, they will display the cached result rather than re-running the entire request. This is not a flaw in the browser processing; its deliberately designed to work that way so as to make GET calls more efficient. A GET call is just retrieving the information; its not meant to change any information on the server, which is why requesting the data again should return the same results. The POST method is for posting or updating information on the server. This type of call is expected to change the data, which is why the results returned from two identical POST calls may very well be completely different from one another. The initial values before the second POST call will be different from the values before the first because the initial call will have updated at least some of those values. A POST call will therefore always obtain the response from the server rather than keep a cached copy of the prior response. How to Choose GET or POST Instead of choosing between GET and POST based on the amount of data you are passing in your Ajax call, you should choose based on what the Ajax call is actually doing. If the call is to retrieve data from the server, then use GET. If the value to be retrieved is expected to vary over time as a result of other processes updating it, add a current time parameter to what you are passing in your GET call so that the later calls will not use an earlier cached copy of the result that is no longer correct. Use POST if your call is going to write any data at all to the server. In fact, you should not only use this criterion for selecting between GET and POST for your Ajax calls but also for when selecting which should be used for processing forms on your web page.

Economic impacts of gambling Essay Example | Topics and Well Written Essays - 1250 words

Economic impacts of gambling - Essay Example To address this issue, gambling agencies and brokers have moved outside of the United States in order to provide an online service, which does not have to respond to the particular legal issues of any one region of the country. With the help of these online gambling websites, the industry has become a major commercial industry, with nearly $335 billion dollars in annual revenue (Leaders). Clearly, there is an economic effect of gambling within the United States; however, the nature and value of this effect is worth consideration. According to Williams, Rehm and Stevens (2011), the research literature examining the effects of gambling on the economy is rich, with over 492 studies addressing the issue alone. Part of this large-scale interest in gambling by economists and other social scientists has to do with the policy decisions that come about constant debate about the worth of casinos and other gambling institutions. While most of these studies are not empirical and look only at the relationship between certain factors like problem gambling and gambling institution proximity, many of these studies do in fact examine the economic issues that arise when a gambling institution is established in a certain locale. ... Gambling tends to benefit government revenue because governments tend to tax casinos more due to the nature of their business and for the fact that profit margins of gambling institutions tend to be higher than any other industry (Leaders). Governments may also benefit from gambling in the sense that they are service provider, such as the case is with lotteries. The profit from these systems is often put into a program or service that receives public support. In Europe, gambling monopolies are held by the government and not private enterprise (Meyer-Arendt and Hartmann). Accordingly, there seems to be a consistency in the results of empirical studies finding that gambling institutions tend to increase the profits of the government at the same time it leads to profits for the industry. Because governments use these profits from gambling, they provide additional public services that would not have been affordable from taxes alone. Governments, as one of the primary beneficiaries, eithe r utilize the public services argument or earmark certain laws in order to maximize their own profit. Thus, gambling can have wide ranges of effects in terms of what the government believes is possible for their public services based on the profits extracted from the gambling industry (Anders). On the other side of the coin, governments may decide to lower taxes instead of providing these public services, which will affect businesses and residents within the jurisdiction of that government (Rankine and Haigh). Any policy related to taxes will eventually affect individual decision-making and incentives. Based on the share of the profits that governments collect from gambling, which can be quite substantial, this effect on taxes

Negotiations - Hypothetical Analysis Essay Example | Topics and Well Written Essays - 2000 words

Negotiations - Hypothetical Analysis - Essay Example Jonathan Brown, and he therefore, is not interested in joining issues with him publicly. He desires that the deliberations be kept absolutely confidential. The hiring policy as envisaged and implemented by Mr. Brown is ideal as per his philosophy but he is out of step with the demands of the time. Mr. Brown’s convictions are right according to him, he has given a sterling medical institution to the public, he is taking care of his staff well, he has no trade union problems, the salaries and perquisites which his staff is paid is significantly higher than what is prevalent in the industry, he treats the staff very well and they adore him as a noble person, and the single point agenda of the Mayor of Boston is proportional representation of the female doctors in the hiring committee. 2. What kind of negotiator is on the other side? The negotiator on the other side is a politician, the Mayor of the city of Boston. Building one’s own power castle is the primary agenda of a politician, there could be rarest of the rare exceptions. By showing his concern for empowering women, he is playing to the gallery. The women employees of the hospital are happy with the administration of Mr. Brown and they are treated very well and get one of the best pay packets in the industry. The negotiator must be having some political compulsions and his desire to discuss the issue with Mr. Brown in confidence indicates that his intentions are not totally insincere. He feels that it is a policy issue and as the Mayor of the city, it is his duty to set right the things. 3. What are your interests? My interests are simple and straightforward. I have been pursuing this policy successfully for the past 35 years. I have no grudge against the womenfolk, absolutely nothing! What I pursue is the principles of the science of ergonomics—finding proper individuals for the proper assignments. I am convinced that women make good nurses, temperamentally they are suitable for that p ost, and I also believe that they make good doctors as well. But there are realistic problems in summoning them to the hospitals in case of emergencies, as women have the primary responsibilities at home. In the overall scheme of administration as envisaged by me, I do not intend to offer them the position of doctors. 4. What are your counterpart’s interests? His concern for the welfare and rights of women seems to be superficial in the present case. He is just counting his votes, and by raising a women-related issue, he is appealing to their emotions with the ultimate aim of converting the sympathy wave into votes. He is visualizing a formidable vote-bank in women and he is planning to turn the tide in his favor. But he is also aware of raking the issues against a reputed hospital, where the presence of the women in the hiring committee is about 16%. (male11, female 2) 5. Who will prepare the agenda and what will the agenda cover? Both the parties to the discussion are seize d about the issue. It seems to be just a one point agenda. A formal agenda has not been circulated either from the side of Mr. Brown or from the side of the Mayor. The discussions will be of a confidential nature, as desired by the Mayor. This shows that the Mayor has some reservations in the back of his mind, and therefore he is insisting for a one to one

Learning Journal Summary Assignment Example | Topics and Well Written Essays - 1250 words

Learning Journal Summary - Assignment Example The main concepts learnt pertains the process of making goals achievable and measurable. Goals keep on pushing us to achieve something, failure to which we lack the direction that we should be taking. I learnt that when setting goals they have to be specific; this helps in narrowing down to a particular goal that one wants to achieve. To make this to be clearly appreciated in the classroom setup we clearly defined our learning objectives. After going through the teachings on setting goals, it became absolutely clear that writing down the goals is not enough in helping us achieve our goals. The specificity of the goal has to be defined based on the specific interventions that can be done in order to ensure that the goal is achieved. In addition, the measure of the goal has to be there in order to monitor progress. I also learnt that the goals set should be evaluated from time to time; the individual or system of evaluation has to be defined. For the successful implementation of the la id down goals, a strategy is necessary in order to achieve them. The strategy acts as the road map that directs the actions of the goal. In order to gain hand-on skills, I participated in the group work deliverable, in which case, I engaged in developing strategies and actions that facilitated an understanding of the topic addressed in the group work. Through the processing of setting up goals in real-life, I was able to meet the challenges that come with establishing and implementing goals. In addition, the learning goal setting provided an opportunity for me to develop self-awareness especially on how I ought to approach goals and the most appropriate strategy of implementing them. The table below shows some of the goals that I set and will use the lessons learnt in this module to successfully implement them. They are achievable, specific, measurable, realistic, and fall within set periods: The second class provided an opportunity to learn more on presentation skills. I have

Event planning: The Southeast Asian Games

Event planning: The Southeast Asian Games Introduction to event The 2015 Southeast Asian Games, officially known as the 28th Southeast Asian Games or 28th Sea Games is a multi-sport event hosted by the city-state of Singapore. Sea games is held once in two years since 1959 and the first time is held in Thailand, Bangkok. The games are under regulation of the Southeast Asian Games Federation with supervision by the International Olympic Committee (IOC) and the Olympic Council of Asia. The games are participated by 11 countries which are Malaysia, Thailand, Singapore, Indonesia, Philippines, Vietnam, Myanmar, Cambodia, Timor-Leste, Brunei and Laos (28th SEA Games, 2015). Singapore is the fourth time has hosted Southeast Asian Games since 1993, after a 22-year long hiatus. The 28th Southeast Asian Games was held from 5th June 2015 until 16th June 2015 at the Singapore Sports Hub as main sporting location. The SEA Games was conceived as an expedient to avail forge vigorous regional cooperation, understanding and unity within the South East Asian community. It was established to be a bi-annual multi-sports event to take place between the years when the Olympic Games and the Asian Games were held. In addition to that, about 7000 athletes from 11 participating nations with 36 sports and 402 events will feature in the 28th Southeast Asian Games Singapore 2015. In 2011 during the 26th Southeast Asian Games, held in Indonesian cities Palembang and Jakarta, as the host of the 28th Southeast Asian Games of 2015, Singapore has been nominated to host the event in 2007 and 2013 before, but turned down both opportunities referring to costs connected with the construction of the new Singapore Sports Hub. The 28th SEA Games will show the people of the Southeast Asia that success ultimately comes to ordinary people with extraordinary dreams. It’s not about having a champion, but inspiring the champion that is inside each of us. Stakeholder of the event People and organisations with a legitimate interest in the outcomes of an event are known as stakeholders. The successful event manager must be able to identify the range of stakeholders in an event and manage their individual needs, which will sometimes overlap and conflict (Johnny et al, 2007). As with event impacts, the event will be judged by its success in balancing the competing needs, expectations and interests of a diverse range of stakeholders. When asked the question ‘what makes an event successful’, there are now numerous shareholders that are key components of modern major events that are looking at a whole range of different measures of success. Thus, the stakeholders that I have chosen for discussion of the event impacts are the government and the sponsorship. The first stakeholders that I have chosen is the organizer of the 28th Southeast Asian Games, which is the Singapore Sports Council (SSC) as it relates to stakeholders of government of Singapore. The Singapore Sports Council (SSC) is the national sports agency responsible for engendering access, opportunities and capabilities for individuals to live better through sports. Through Vision 2030, Singapore Sports Council seeks to employ sports as a national strategy to stimulate positive, deeply embedded values for Singaporeans and Singapore. Singapore Sports Council was founded in 1973, through the merger of the National Sports Promotion Board (NSPB) and the National Stadium Corporation (NSC). It engenders opportunities for people to excel in sports; opportunities for people to be engaged in sports as, coaches, officials, volunteers or fans and opportunities for people to do business in sports as sponsors and investors. Singapore is a sovereign republic, with a legal system based on the English common law. The Constitution lays down the fundamental principles and basic framework for the three organs of state, namely, the Executive, the Legislative and the Judiciary. The Executive comprises the Cabinet, which is responsible for the general direction of the Government and accountable to Parliament. The Legislature comprises the Parliament and is the legislative authority responsible for enacting legislation. More information on the history of Parliament, Parliament House and activities of the House can be obtained from its website. The Judiciarys function is to independently administer justice. The Judiciary is safeguarded by the Constitution. The Prime Minister of Singapore is appointed by the President of Singapore under Article 25 of the Constitution. The President, acting on the advice of the Prime Minister, also appoints other Ministers from among the Members of Parliament. The Prime Minister is the effective head of the executive branch of government. He chairs the Cabinet, which is the central decision-making body of the executive government. It is an organ of state and central to Singapores system of government. Click for more information on the Cabinet appointments. In order to attract sponsorships, event managers must offer tangible benefits to sponsors, and effective programs to deliver them. Large corporations such as Coca-Cola, 100 Plus and Telstra receive hundreds of sponsorship applications each week, and only consider those events that have a close fit with corporate objectives and a demonstrable ability to deliver benefits. For the event, sponsorship is therefore much more than a means of boosting revenues, but must also meet the needs and objectives of its sponsors (William et al, 2007). Therefore, the second stakeholders that I have chosen is the main sponsor of 28th Southeast Asian Games, which is the NTUC FairPrice and NTUC FairPrice Foundation. NTUC Fairprice Co-operative Ltd was founded by the labour movement in 1973, with a social mission to moderate the cost of living in Singapore. From one supermarket, it has grown to become Singapore’s largest retailer serving about 430,000 shoppers daily, with a network of over 120 outlets, comprising FairPrice supermarkets, FairPrice Finest and FairPriceXtra. Its convenience stores, FairPrice Xpress and Cheers, has a network spanning over 160 convenience stores island wide and serves over 100,000 customers daily. NTUC FairPrice also owns a Fresh Food Distribution Centre and a centralised warehousing and distribution company. Today, with its multiple retail formats serving the varied needs and interests of people from all walks of life, NTUC FairPrice has kept pace with the changing needs of its customers while remaining committed to its social mission and its aspiration to be Singapore’s leading world-class retailer with a heart. Guided by the philosophy to do well in order to do good for the community, NTUC FairPrice set up the FairPrice Foundation in 2008 to focus its giving efforts to provide a better life for the community. FairPrice Foundation focuses its giving on three strategic thrusts – the poor and needy, nation building and community bonding, and advancing workers’ welfare. FairPrice has since donated $76 million to the Foundation to further these three areas of focus. Political benefits of the event towards chosen stakeholder Based on the details above, political benefits towards NTUC FairPrice and NTUC FairPrice Foundation is increase international prestige. In Singapore, NTUC FairPrice’s sponsorship of 28th Sea Games gave it the benefits of getting its brand name quickly recognised in the Singapore market. It is important for event managers to identify exactly what NTUC FairPrice want from 28th Sea Games and what the 28th Sea Games can deliver for them. They may be seeking mechanisms to drive sales, or want to strengthen client relationships through 28th Sea Games to increase their international prestige and to attract more new customer. Besides, political benefits towards NTUC FairPrice and NTUC FairPrice Foundation and the government of Singapore is promoting social cohesion. The sponsorship will include SGD $2 million cash from FairPrice Foundation and SGD $3 million value in kind from NTUC FairPrice to the 28th Sea Games in support of the region’s most prestigious multisport event. This is the largest donation FairPrice and FairPrice Foundation have made to a sporting event to date. This is an ideal partnership for the organizer of 28th SEA Games which are Singapore Sports Council (SSC) as NTUC FairPrice has a strong relationship with the community. As part of FairPrice Foundation’s aim to create a better life for the community, the Foundation supports major activities and social community projects that cater to Singaporeans at large, to foster closer relationships among the community. Economic benefits of the event towards chosen stakeholder A primary concern of an event entrepreneur or host organisation is whether an event is within budget and, hopefully, results in a surplus or profit. This is a simple matter of whether the income from sponsorship, merchandise and ticket sales exceeds the costs of conducting and marketing the event. However, from the perspectives of the host communities and governments, a wider range of economic impacts is often of equal or greater significance. Therefore, economic benefits towards government are business opportunities and employment creation. Events can provide their host communities with a strong platform for showcasing their expertise, hosting potential investors and promoting new business opportunities. During the 28th SEA Games, the government of Singapore spent Environmental benefits of the event towards chosen stakeholder A major event may require an environmental impact assessment before council permission is granted for it to proceed. Even if a formal study is not required, the event manager should carefully consider the likely impact of the event on the environment. This impact will be fairly contained if the event is to be held in a suitable purpose-built venue, for example, a stadium, sportsground, showground or entertainment centre, but may be much greater if the event is to be held in a public space not ordinarily reserved for events such as a park, town square or street. Crowd movement and control, noise levels, access and parking will often be important considerations. Therefore, environmental benefits towards government is increased environmental awareness and urban transformation and renewal. Firstly, environmental benefits towards government is increased environmental awareness. Governments of Singapore in 28th Sea Games, are increasingly using public education and legislation to promote the recycling of waste materials and reduce the amount of waste going to landfill. 28th Sea Games are targeted as opportunities to demonstrate best practice models in waste management and to change public attitudes and habits. Incorporating a waste management plan into the overall event plan has become increasingly good policy. Audience of the 28th Sea Games expectations and the health of the environment require that events demonstrate good waste management principles and provide models for recycling. The waste-wise event manager (government) will reap not only economic benefits, but also the approval of an increasing environmentally aware public. Secondly, environmental benefits towards government is urban transformation and renewal. In the staging of large events in Singapore such as this 28th Sea Games, the provision of infrastructure is often a costly budget component, but this expenditure usually results in an improved environment and facilities for Singapore, and provides a strong incentive for Singapore to act as host. The government of Singapore has been constructed the original stadium to a new sport complex, which is the Singapore Sports Hub and it will be Singapores premier land and sea sports, entertainment and lifestyle destination. The Singapore Sports Hub is a state-of-the-art, fully integrated sports, entertainment and lifestyle hub that will allow everyone in Singapore to watch, play and support sports and entertainment. Featuring a unique cluster development of integrated world-class sports facilities within the city, it will not only spur the development of the sports industry, excellence and participation, but also take sports to the next level in Singapore. Other Related Information Reference List 28th SEA Games. Available from: https://www.seagames2015.com > NTUC FairPrice. Available from: www.fairprice.com.sg > Government Singapore. Available from: www.fairprice.com.sg > Johnny Allen, William O’Toole, Robert Harris, Ian McDonnell, 2007. Festival Special Event Management, Irwin, Sydney. Jonathan Wong, 2015. Singapore SEA Games Budget. Available from: http://www.gov.sg/government/web/content/61466480471bdd139c90dc5660466bae/20150129_ST_SporesSEAGamesbudget3245m.pdf?MOD=AJPERESuseDefaultText=0useDefaultDesc=0> Gene Deletion Strategy: Identify Function of Non-Coding RNA Gene Deletion Strategy: Identify Function of Non-Coding RNA Molecular Biology mainly Non coding RNA Project Contents (Jump to) Non coding RNA Small Nuclear RNA genes Materials and Methods Media YPD and YPD+G418 plates Strain confirmation References A Gene Deletion Strategy to Identify the Function of Non-Coding RNA in the Eukaryotic Genome, Using the Model Organism Saccharomyces cerevisiae Abstract The yeast Saccharomyces cerevisiae is a key model organism for investigating cellular processes. While it is important to determine the role protein-coding genes play in biological, it is now clear that RNA also plays an important role in these cellular processes. Deletion cassettes were constructed successfully by a PCR based strategy for 12 ncRNAs, which are snR19, snR20, snR14, snR6, snR7, TLC1, SCR1, NME1, RPR1, RUF20, RUF21, and RUF22. Deletions cassettes were transformed into the diploid reference strain BY4743 and the ncRNA gene replaced with the KanMX marker byhomologous recombination. Heterozygous deletion strains were confirmed for each of the 12 genes by extensive PCR analysis and showed positive results for the insertion of the cassette. Following sporulation and tetrad dissection to segregate haploid spores and growth on different selective media, 10 of the 12 ncRNAs were shown to be essential for cell survival by producing 2 viable spores. These include snR19, snR20, sn R14, snR6, snR7, TLC1, SCR1, NME1, RPR1 and RUF20.However, RUF21 and RUF 22 produced four viable haploid spores indicating that these are non-essential genes for the cell.This resource will now enable the role of ncRNAs in cellular processes to be determined, by growth of these deletion strains in a range of different conditions and in different genetic backgrounds. Non coding RNA The term non-coding RNA (ncrna) is usually utilized for RNA that does not encode a protein, however this does not imply that such Rnas dont contain data nor have capacity. In spite of the fact that it has been by and large accepted that most hereditary data is transacted by proteins, late proof proposes that most of the genomes of vertebrates and other complex life forms is indeed translated into ncrnas, a considerable lot of which are alternatively joined and/or transformed into more diminutive items. These ncrnas incorporate micrornas and snornas (numerous if not a large portion of which stay to be distinguished), and likely different classes of yet-to-be-found little administrative Rnas, and a huge number of longer transcripts (counting complex examples of intertwining and covering sense and antisense transcripts), the vast majority of whose capacities are obscure. These Rnas (counting those inferred from introns) seem to embody a shrouded layer of internal signs that control diff erent levels of quality articulation in physiology and advancement, including chromatin structural engineering/epigenetic memory, translation, RNA grafting, altering, interpretation and turnover. RNA administrative systems may focus a large portion of our complex attributes, assume a critical part in ailment and constitute an unexplored universe of hereditary variety both inside and between species. (Mattick JS, 2006) In this project, the functionality of the following non-coding RNA genes in S.cerevisiae will be determined. The non-coding RNA genes mentioned in this study have significant importance in the yeast genome and the determination of functionality of these genes will unveil their exact contribution to eukaryotic cellular function. Non coding RNA genes are those that are transcribed but not translated. They code for either long (>200) or short (200et al., 2012). The highly abundant short non coding RNA genes have been well characterized and are known to play an important role in various cellular functions such as transcriptional regulation, translation regulation, RNA editing etc. Both long and short non coding RNAs are mostly transcribed by RNA polymerase II. However, some species of non coding RNAs are also transcribed by RNA pol III and I. Small Nuclear RNA genes The review article by Guthrie explains the steps that were undertaken to identify the snRNAs associated with the spliceosome (Guthrie and Patterson, 1988) and work carried out by the same group revealed that many of the ‘U-RNAs’ were encoded by single-copy genes, such as U5 which is encoded by snR7 (Riedel et al., 1986). ‘U’ in RNA stand for Uracil, each nucleotide in RNA encloses a ribose sugar, with carbons numbered 1 throughout 5. A base is appended to the 1 position, all in all, cytosine (C), adenine (A), uracil (U), or guanine (G). Cytosine and uracil are pyrimidines, Adenine and guanine are purines. Materials and Methods Media YPD and YPD+G418 plates YPD media were made from 2% D-glucose, 1% Bacto yeast extract, 2% Bacto-peptone. YPD plates were prepared by adding 2% agar to the YPD media. Agar (2%) and G418 Geneticin (200Â µg/ml) was supplement in the YPD media for the preparation YPD+G418 plates. The recepie for YPD+G418 plates is: Bacto Peptone Difco 10g Bacto Yeast Extract Difco 10g Glucose 20g Bacto Agar (if plates) 20g Distilled water qsp 1L Autoclave at 0,5bar After autoclavage let cool down until 55Â °C Add G418 to have a final concentration of 200mg/L (1 mL of 200mg/mL G418 in 1L of YPD) (Genetics of Intra Specie Variations) Identification of mating types and cross mating Using 96 well plate, colonies of G418 resistance spores, which have been isolated from replica platting method, were inoculated in 100ul of YPD and 7ulof DMSO and incubated overnight at 30Â °C. This stock can be stored at -20Â °C. The RT2 a strain and RT3 ÃŽ ± strain were allowed to grow in 5ml YPD culture overnight at 30Â °C. Following that two separated 96 well plates were used by filling each well with 10Â µl of the RT2 a strain or RT3 ÃŽ ± strain separately. Then 10Â µl of the tested samples were added each well of the known strains. These were then incubated overnight at 30oC. Following incubation, metal frogger was used to replica plate the crosses onto deficiency plates without leucine and tryptophan. The incubation period was allowed until the colonies were able to be obtained on the deficient media. For crossing, 2ul of the stock spores were pipetted on to deficiency plates without methionine and deficient plates without lysine. This was incubated at 30Â °C until the growth was determined. When all data of the deficiency plates and mating types was known, cross spores of opposite mating type and one defficiency was done by adding 10ul of each mating stock culture into an eppendrof tube and incubated overnight at 30Â °C. Strain confirmation To confirm that the picked cells were haploid spores and that the cells were originally BY4743 yeast, all dissected spores were grown on different SD media. RUF22 G418 -URA -HIS -LEU LYS -MET 1A + + + 1B + 1C 1D + + 2A + + + 2B + + 2C 2D + 3A + + 3B + 3C + 3D + + 4A + + 4B 4C + + 4D + + 5A + 5B + 5C + 5D + + + 6A + + 6B + + 6C + 6D + 7A + 7B + 7C + + 7D + + Table 2: Growth of the deletion strain tetrads on different SD media for RUF22 gene. Each colony was scored for significant growth (+) or no/poor growth (-) on each medium. RUF21 G418 -URA -HIS -LEU LYS -MET 1A + 1B + 1C + + 1D + + 2A + 2B + + 2C + + 2D + 3A + + 3B + 3C + 3D + + 4A + + 4B + + 4C + + 4D 5A + 5B + + 5C 5D + + + 6A + + 6B + + 6C + 6D + Table 3: Growth of the deletion strain tetrads on different SD media for RUF21 gene. Each colony was scored for significant growth (+) or no/poor growth (-) on each medium. TLC1 G418 -URA -HIS -LEU LYS -MET 1A + + 1B + + 1C 1D + + 2A + + 2B + + + 2C 2D + 3A + + + 3B + 3C + 3D + 4A + + 4B + 4C + 4D + + 5A 5B + + 5C + 5D + + + 6A + + 6B + + 6C 6D + + 7A 7B 7C + + + 7D + + + Table 4: Growth of the deletion strain tetrads on different SD media for TL gene. Each colony was scored for significant growth (+) or no/poor growth (-) on each medium. The data in Tables 2 and 3 shows the results of the dissected RUF22 and RUF21 spores after being given the required time to grow. It was found that there was no growth in the SD(-URA), SD(-HIS) and SD(-LEU) plates indicating that they were BY4743-derived tetrads. Moreover, the growth of the spores observed in the YPD+G418, SD(-MET) and SD(-LYS) in the case of RUF22 and RUF21 deleted strains indicating that these cells derived from tetrads and were haploid knockouts. References Genetics of Intra Specie Variations. (n.d.). Retrieved September 19, 2014, from http://www.ens-lyon.fr/LBMC/gisv/index.php/en/protocols/yeast-methods/72-yeast-media-recipes Mattick JS, M. I. (2006, April 15). Pub Med. Retrieved September 19, 2014, from http://www.ncbi.nlm.nih.gov/pubmed/16651366